Biochemical changes in some banana cultivars infected by Banana bunchy top virus
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Abstract
Bunchy top is one of the major diseases on bananas that caused economic losses. This study aimed to determine changes in protein content, the concentration of phenolic compounds, the amount of chlorophyll, and sugar content in several Banana bunchy top virus (BBTV)-infected banana cultivars. The research involved the artificial inoculation, extraction, and biochemical analysis of banana seedlings from the cultivars Mas, Cavendish, Kepok, and Raja. Inoculation was performed using the vector Pentalonia sp.. A factorial complete randomized design (CRD) was used as the experimental design. Each experimental unit consisted of 5 repetitions. Protein, phenol, chlorophyll, and sugar content measurements were performed using a UV-Vis spectrophotometer. Test samples were in the form of fresh leaves from BBTV-infected and non-BBTV-infected plants. Regression analysis and tests for significance were performed to determine the biochemical changes that occurred after testing. The results showed that the average levels of phenols, chlorophyll, protein, and total sugars in BBTV-infected plants were significantly altered. The chlorophyll content decreased from 82–87% in Mas, Cavendish, and Raja cultivars. Total plant phenol in all BBTV-infected cultivars increased by 69.2 to 348.3 ppm. Specificity was evident in the change in protein concentration, which increased by 95 ppm and 84.5 ppm in the Mas and Cavendish cultivars, respectively. This contrasts with the Kepok and Raja cultivars, which decreased by 660.5 ppm and 113.6 ppm, respectively. Sugar levels increased from 3133.9 to 3298.6 ppm in all BBTV-infected breeds. According to the data, BBTV infection has been shown to cause physiological and biochemical changes in Mas, Cavendish, Kepok, and Raja.
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References
Arumugam C, Kalaimughilan K, & Kathithachalam A. 2017. Banana bunchy top viral coat protein (CP) gene expression studies at molecular level in hill banana cv. Sirumalai (AAB). Intl. J. Curr. Microbiol. App. Sci. 6(6): 398–411. https://doi.org/10.20546/ijcmas.2017.606.047
Anuradha C & Selvarajan R. 2021. Biochemical alterations in banana cultivars infected systemically by Banana bract mosaic virus (BBrMV). J. Pharmacogn. Phytochem. 10(1): 2619–2627. https://doi.org/10.22271/phyto.2021.v10.i1ak.13755
Anuradha C, Selvarajan R, Vasantha S, & Suresha GS. 2015. Biochemical characterization of compatible plant virus interaction: A case study with Bunchy top virus-banana host-pathosystem. Plant Pathol. J. 14(4): 212–222. https://doi.org/10.3923/ppj.2015.212.222
Bahar T, Qureshi AM, Qurashi F, Abid M, Zahra MB & Haider MS. 2020. Changes in phyto-chemical status upon viral infections in plant: A critical review. Phyton. 90(1): 75–86. https://doi.org/10.32604/phyton.2020.010597
Baldodiya GM, Baruah G, Borah BK, Modi MK, & Nath PD. 2019. Molecular characterization and sequence analyses of Banana bunchy top virus infecting banana cultivar Jahaji (Dwarf Cavendish) in Assam, India. 3 Biotech 9: 110. https://doi.org/10.1007/s13205-019-1636-5
Bressan A & Watanabe S. 2011. Immunofluorescence localisation of Banana bunchy top virus (family Nanoviridae) within the aphid vector, Pentalonia nigronervosa, suggests a virus tropism distinct from aphid-transmitted luteoviruses. Virus Res. 115(2): 520–525. https://doi.org/10.1016/j.virusres.2010.12.005
Debbarma R, Kumar KK, Sudhakar D, & Soorianathasundaram K. 2019. Molecular characterization of Banana bunchy top virus movement protein-encoding DNA-M component isolated from hill banana and Grand Naine. Electr. J. Plant Breed. 10(2): 936–943. https://doi.org/10.5958/0975-928X.2019.00120.0
Dong X, Xiong Y, Ling N, Shen Q, & Guo S. 2014. Fusaric acid accelerates the senescence of leaf in banana when infected by Fusarium. World J. Microb. Biot. 30(4): 1399–1408. https://doi.org/10.1007/s11274-013-1564-1
Fadhlullah HR, Rusmiyanto PWE, & Zakiah Z. 2020. Respon morfologi, biomassa, dan kandungan klorofil daun mimosa air (Neptunia oleracea L.) pada air yang terpapar merkuri klorida (HgCl2) [Morphological response, biomass and chlorophyll content of water mimosa (Neptunia oleracea L.) leaves in water exposed by mercury (HgCl2)]. Protobiont. 9(3): 206–213.
Furuya N, Dizon TO, & Natsuaki KT. 2006. Molecular characterization of Banana bunchy top virus and Cucumber mosaic virus from Abaca (Musa textilis Nee). Jour. Agri. Sci. 51(2): 92–101.
Hasan K. 2022. Determination of protein content by spectrophotometric method and fat content by soxhletation method on purple kopek eggplant and green kopek eggplant. JUMPA. 2(2): 102–107.
Hooks CRR, Wright MG, Kabasawa DS, Manandhar R, & Almeida RPP. 2008. Effect of Banana bunchy top virus infection on morphology and growth characteristics of banana. Ann. Appl. Biol. 153(1): 1–9. https://doi.org/10.1111/j.1744-7348.2008.00233.x
Jeandet P, Formela-Luboi?ska M, Labudda M, & Morkunas I. 2022. The role of sugars in plant responses to stress and their regulatory function during development. Int. J. Mol. Sci. 23(9): 5161. https://doi.org/10.3390/ijms23095161
Jeandet P, Vannozzi A, Sobarzo-Sánchez E, Uddin MdS, Bru S, Martínez-Márquez A, Clément C, Cordelier S, Manayi A, Nabavi SF, Rasekhian M, Batiha GES, Khan H, Morkunas I, Belwal T, Jiang J, Koffas M, & Nabavi SM. 2021. Phytostilbenes as agrochemicals?: biosynthesis, bioactivity, metabolic engineering and biotechnology. Nat. Prod. Rep. 38: 1282–1329. https://doi.org/10.1039/d0np00030b
Ji XL, Yu NT, Qu L, Li BB, & Liu ZX. 2019. Banana bunchy top virus (BBTV) nuclear shuttle protein interacts and re-distributes BBTV coat protein in Nicotiana benthamiana. 3 Biotech 9: 121. http://doi.org/10.1007/s13205-019-1656-1
Leiwakabessy M, Nurulita S, & Hidayat SH. 2017. Disease incidence and molecular analysis of Banana bunchy top virus in Bogor, West Java. In: Efendi D & Maharijaya A (Eds.). The Future of Tropical Horticulture. International Proceeding on Tropical Horticulture 2016: The Future of Tropical Horticulture. Bogor, Indonesia.
Kaur S, Samota MK, Choudhary M, Choudhary M, Pandey AK, Sharma A, & Thakur J. 2022. How do plants defend themselves against pathogens-Biochemical mechanisms and genetic interventions. Physiol. Mol. Biol. Plants. 28(2): 485–504. https://doi.org/10.1007/s12298-022-01146-y
Kumar PL, Selvarajan R, Iskra-Caruana ML, Chabannes M, & Hanna R. 2015. Biology, etiology, and control of virus diseases of banana and plantain. In: Loebenstein G & Katis NI (Eds.). Advances in Virus Research. Vol. 91. pp. 229–269. Academic Press. https://doi.org/10.1016/bs.aivir.2014.10.006
Kumar S, Abedin MdM, Singh AK, & Das S. 2020. Role of phenolic compounds in plant-defensive mechanisms. In: Lone R, Shuab R, & Kamili AN (Eds.). Plant Phenolics in Sustainable Agriculture. Vol. 1. pp. 517–532. Springer Singapore. https://doi.org/10.1007/978-981-15-4890-1
Madhumitha B, Karthikeyan A, Devi GP, Aiyanathan KEA, & Sudha M. 2020. Comparative evaluation of biochemical changes in the leaves of resistant and susceptible mungbean plants infected by Mungbean yellow mosaic virus. Res. J. Biotechnol. 15(2): 47–54.
Mahfouze HA, El-Dougdoug NK, & Mahfouze SA. 2020. Virucidal activity of silver nanoparticles against Banana bunchy top virus (BBTV) in banana plants. Bull. Natl. Res. Cent. 44: 199. https://doi.org/10.1186/s42269-020-00433-6
Musidlak O, Nawrot R, & Go?dzicka-Józefiak, A. 2017. Which plant proteins are involved in antiviral defense? Review on in vivo and in vitro activities of selected plant proteins against viruses. Int. J. Mol. Sci. 18(11): 2300. https://doi.org/10.3390/ijms18112300
Pandey MK & Singh A. 2019. Total phenols and sugar content in wheat resistant and susceptible cultivars against karnal bunt. Plant Arch. 19(1): 101–103.
Paudel DB & Sanfaçon H. 2018. Exploring the diversity of mechanisms associated with plant tolerance to virus infection. Front. Plant Sci. 9: 1575. https://doi.org/10.3389/fpls.2018.01575
Qazi J. 2016. Banana bunchy top virus and the bunchy top disease. J. Gen. Plant Pathol. 82(1): 2–11. https://doi.org/10.1007/s10327-015-0642-7
Rahayuniati RF, Hartono S, Somowiyarjo S, Subandiyah S, & Thomas JE. 2021. Characterization of Banana bunchy top virus on Sumatra (Indonesia) wild banana. Biodiversitas. 22(3): 1243–1249. https://doi.org/10.13057/biodiv/d220321
Rahayuniati RF & Subandiyah S. 2022. Symptom expression and resistance of some banana cultivars to Banana bunchy top virus infection. Agr. Nat. Resour. 56(5): 1019–1028. https://doi.org/10.34044/j.anres.2022.56.5.16
Rodriguez-Peña R, Mounadi KE, & Garcia-Ruiz H. 2021. Changes in subcellular localization of host proteins induced by plant viruses. Viruses. 13(4): 677. https://doi.org/https://doi.org/10.3390/v13040677
Siddique Z, Akhtar KP, Hameed A, Sarwar N, Ul-Haq I, & Khan SA. 2014. Biochemical alterations in leaves of resistant and susceptible cotton genotypes infected systemically by Cotton leaf curl Burewala virus. J. Plant Interact. 9(1):702–711. https://doi.org/10.1080/17429145.2014.905800
Souza PFN, Garcia-Ruiz H, & Carvalho FEL. 2019. What proteomics can reveal about plant–virus interactions? Photosynthesis-related proteins on the spotlight. Theor. Exp. Plant Physiol. 31(1): 227–248. https://doi.org/10.1007/s40626-019-00142-0
Teixeira RSS, da Silva ASA, Ferreira-Leitão VS, & Bon EPdS. 2012. Amino acids interference on the quantification of reducing sugars by the 3,5-dinitrosalicylic acid assay mislead carbohydrase activity measurements. Carbohydr. Res. 363: 33–37. https://doi.org/10.1016/j.carres.2012.09.024
Tanuja N, Ramanathan A, Vanitha S, Soorianathasundaram K, & Kumar KK. 2019. Differential biochemical response among banana (Musa spp.) genotypes against Banana bunchy top virus (BBTV). CJAST. 38(6): 1–11. https://doi.org/10.9734/CJAST/2019/v38i630416
Wallis CM & Galarneau ERA. 2020. Phenolic compound induction in plant-microbe and plant-insect interactions: A meta-analysis. Front. Plant Sci. 11: 580753. https://doi.org/10.3389/fpls.2020.580753
Waterborg JH & Matthews HR. 1984. The Lowry method for protein quantitation. In: Walker JM (Ed.). Proteins. Methods in Molecular Biology, Vol 1. pp. 7–9. Humana Press. New Jersey. https://doi.org/10.1385/0-89603-062-8:1
Wickramaarachchi WART, Shankarappa KS, Rangaswamy KT, Maruthi MN, Rajapakse RGAS, & Ghosh S. 2016. Molecular characterization of Banana bunchy top virus isolated from Sri Lanka and its genetic relationship with other isolates. VirusDis. 27(2): 154–160. https://doi.org/10.1007/s13337-016-0311-2
Yang H & Luo P. 2021. Changes in photosynthesis could provide important insight into the interaction between wheat and fungal pathogens. Int. J. Mol. Sci. 22(16): 8865. https://doi.org/10.3390/ijms22168865
Zhao J, Zhang X, Hong Y, & Liu Y. 2016. Chloroplast in plant-virus interaction. Front. Microbiol. 7: 1565. https://doi.org/10.3389/fmicb.2016.01565